anhydrous dmf Search Results


anhydrous n n dimethylformamide  (Vector Laboratories)
95
Vector Laboratories anhydrous n n dimethylformamide
Table 3
Anhydrous N N Dimethylformamide, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf  (Chem Impex International)
95
Chem Impex International anhydrous dmf
Table 3
Anhydrous Dmf, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf  (Corning Life Sciences)
90
Corning Life Sciences anhydrous dmf
Table 3
Anhydrous Dmf, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf (99.5)  (Fluka Chemical)
90
Fluka Chemical anhydrous dmf (99.5)
Table 3
Anhydrous Dmf (99.5), supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf  (FUJIFILM)
90
FUJIFILM anhydrous dmf
Table 3
Anhydrous Dmf, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf  (Boron Molecular)
90
Boron Molecular anhydrous dmf
Table 3
Anhydrous Dmf, supplied by Boron Molecular, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n -hydroxybenzotriazole (hobt)  (Fluorochem Ltd)
90
Fluorochem Ltd n -hydroxybenzotriazole (hobt)
Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and <t>DIC/HOBt;</t> (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).
N Hydroxybenzotriazole (Hobt), supplied by Fluorochem Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dimethylformamide (dmf  (FUJIFILM)
90
FUJIFILM anhydrous dimethylformamide (dmf
Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and <t>DIC/HOBt;</t> (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).
Anhydrous Dimethylformamide (Dmf, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf for peptide synthesis  (RCI Labscan Ltd)
90
RCI Labscan Ltd anhydrous dmf for peptide synthesis
Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and <t>DIC/HOBt;</t> (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).
Anhydrous Dmf For Peptide Synthesis, supplied by RCI Labscan Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf  (Fisher Scientific)
90
Fisher Scientific anhydrous dmf
Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and <t>DIC/HOBt;</t> (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).
Anhydrous Dmf, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous dmf  (Kanto Chemical)
90
Kanto Chemical anhydrous dmf
Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and <t>DIC/HOBt;</t> (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).
Anhydrous Dmf, supplied by Kanto Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anhydrous n,n-dimethylformamide (dmf)  (Kanto Chemical)
90
Kanto Chemical anhydrous n,n-dimethylformamide (dmf)
Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and <t>DIC/HOBt;</t> (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).
Anhydrous N,N Dimethylformamide (Dmf), supplied by Kanto Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Table 3

Journal: Journal of visualized experiments : JoVE

Article Title: Reusable Single Cell for Iterative Epigenomic Analyses

doi: 10.3791/63456

Figure Lengend Snippet: Table 3

Article Snippet: Anhydrous DMF , Vector laboratories , S-4001-005 , Anhydrous N,N-dimethylformamide (DMF).

Techniques: Staining, Fluorescence, Microscopy, Plasmid Preparation, Bioburden Testing, Clone Assay, Ligation, Cell Counting, Spectrophotometry, Purification, Electron Microscopy, Transferring, Gel Extraction, Picogreen Assay, Recombinant

Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and DIC/HOBt; (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).

Journal: Pharmaceutics

Article Title: Linker-Free Synthesis of Antimicrobial Peptides Using a Novel Cleavage Reagent: Characterisation of the Molecular and Ionic Composition by nanoESI-HR MS

doi: 10.3390/pharmaceutics15041310

Figure Lengend Snippet: Scheme of the synthesis of polymyxin B 3 ( n -octyl-Dab-Thr-Dab- cyclo [Dab-Dab-DPhe-Leu-Dab-Dab-Thr]. (i) 1.2 eq of Fmoc-Thr(Bn)-OH and 4 eq of DIPEA in CH 2 Cl 2 at room temperature, overnight; (ii) 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (iii) repetitive cycles of coupling and deprotection using a 3-fold molar excess of protected Fmoc-AA-OH (or octanoic acid at the end) and DIC/HOBt; (iv) CH 2 Cl 2 /TFA/TES/H 2 O (55:40:3:2) for 30 min at room temperature (ŋ cleavage = 82%); (v) HATU/HOAt/DIPEA (2:2:4 eq) for 2–4 h, monitored by TLC stained with ninhydrin (ŋ cyclization = 81%); and (vi) HBr/Et 3 Si-Br/TES/TFA, in situ prepared with TFA/TES/Br 2 (82.5:15:2.5) for 45 min at room temperature (ŋ acidolysis = 92%). Final yield: 21%, purity > 95%. R = C 7 H 15 (corresponding to octanoyl).

Article Snippet: 4-Methylbenzhydrylamine hydrochloride resin (MBHA), 2-Chlorotrityl chloride resin (2-CTC), N -fluorenylmethoxycarbonyl (Fmoc)-protected amino acids, trifluoroacetic acid (TFA), and N -hydroxybenzotriazole (HOBt) were purchased from Fluorochem (Hadfield, UK) and Iris Biotech GmbH (Marktredwitz, Germany).

Techniques: Staining, In Situ

Complete scheme of the synthetic route of ( A ) dusquetide; ( B ) segments 8–14 of RR4. (i) Repetitive cycles of coupling and deprotection using a 3-fold molar excess of Fmoc-AA-OH/DIC/HOBt and 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (ii) TFA/HBr/Et 3 SiBr/TES, obtained by reaction at 0 °C of TFA:TES:Br 2 (82.5:15:2.5) for 90 min at room temperature; (ii*) TFA/HBr/iP 3 SiBr/TIS, obtained by reaction at 0 °C of TFA:TIS:Br 2 (82.5:15:2.5) for 90 min at room temperature.

Journal: Pharmaceutics

Article Title: Linker-Free Synthesis of Antimicrobial Peptides Using a Novel Cleavage Reagent: Characterisation of the Molecular and Ionic Composition by nanoESI-HR MS

doi: 10.3390/pharmaceutics15041310

Figure Lengend Snippet: Complete scheme of the synthetic route of ( A ) dusquetide; ( B ) segments 8–14 of RR4. (i) Repetitive cycles of coupling and deprotection using a 3-fold molar excess of Fmoc-AA-OH/DIC/HOBt and 20% of piperidine in DMF (1 × 1 min, 2 × 10 min); (ii) TFA/HBr/Et 3 SiBr/TES, obtained by reaction at 0 °C of TFA:TES:Br 2 (82.5:15:2.5) for 90 min at room temperature; (ii*) TFA/HBr/iP 3 SiBr/TIS, obtained by reaction at 0 °C of TFA:TIS:Br 2 (82.5:15:2.5) for 90 min at room temperature.

Article Snippet: 4-Methylbenzhydrylamine hydrochloride resin (MBHA), 2-Chlorotrityl chloride resin (2-CTC), N -fluorenylmethoxycarbonyl (Fmoc)-protected amino acids, trifluoroacetic acid (TFA), and N -hydroxybenzotriazole (HOBt) were purchased from Fluorochem (Hadfield, UK) and Iris Biotech GmbH (Marktredwitz, Germany).

Techniques: